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Enterovirus 71 VP2 antibody

Anti-Enterovirus 71 VP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132340
Anti-Enterovirus 71 VP2 antibody used in Western Blot (WB). GTX132340
Anti-Enterovirus 71 VP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132340

Cat No. GTX132340

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Enterovirus 71
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of EV71 VP2 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Enterovirus 71 VP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132340

GTX132340 ICC/IF Image

EV71 VP2 antibody detects EV71 VP2 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP2 protein stained by EV71 VP2 antibody (GTX132340) diluted at 1:500. Blue: DAPI staining. Scale bar = 500 μm.

Anti-Enterovirus 71 VP2 antibody used in Western Blot (WB). GTX132340

GTX132340 WB Image

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP2 antibody (GTX132340) diluted at 1:5000.

Anti-Enterovirus 71 VP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132340

GTX132340 IHC-P Image

EV71 VP2 antibody detects EV71 VP2 protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 VP2 antibody [GTX132340] diluted at 1:500
Antigen Retrieval: Citrate buffer

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf