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Enterovirus 71 VP3 antibody

Anti-Enterovirus 71 VP3 antibody used in Western Blot (WB). GTX132341
Anti-Enterovirus 71 VP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132341

Cat No. GTX132341

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Enterovirus 71
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

EV71-infected RD cell
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of EV71 Vp3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Enterovirus 71 VP3 antibody used in Western Blot (WB). GTX132341

GTX132341 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EV71 VP3 antibody (GTX132341) diluted at 1:1000.

Anti-Enterovirus 71 VP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132341

GTX132341 ICC/IF Image

EV71 VP3 antibody detects EV71 VP3 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP3 protein stained by EV71 VP3 antibody (GTX132341) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.

REFERENCE
There are currently no references for Enterovirus 71 VP3 antibody (GTX132341). Be the first to share your publications with this product.
REVIEW

Enterovirus 71 VP3 antibody Cat No. GTX132341

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : WB
Sample Species : EV71
Sample : EV71 infected vero cells
Amount used : 6μg
Blocking : 5% milk in PBST, 25°C, 4Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: EV71 infected vero cells.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf