Enterovirus 71 antibody

Name: Enterovirus 71 antibody
Brand: GeneTex
SKU: GTX124261
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Enterovirus 71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124261

Anti-Enterovirus 71 antibody used in IHC (Paraffin sections) (IHC-P). GTX124261

Anti-Enterovirus 71 antibody used in Western Blot (WB). GTX124261

Cat. No. GTX124261

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Enterovirus 71
Package	100 μl ($399), 25 μl ($169)

See all Enterovirus 71 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
ELISA	Assay dependent

Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

The immunogen used to generate this antibody corresponds to EV71

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_11161990

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Enterovirus 71 , EV71

Research Area

DATA IMAGES

GTX124261 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed EV71 infected Vero cell, using EV71 (GTX124261) antibody at 1:1000 and 1:5000 dilution.

GTX124261 IHC-P Image

EV71 antibody detects EV71 viral protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 antibody [GTX124261] diluted at 1:500
Antigen Retrieval: Citrate buffer

GTX124261 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Enterovirus 71 antibody (GTX124261) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124261 WB Image

EV71 antibody detects EV71 protein by western blot analysis. Un-infected (-) and infected with EV71 C2 or B4 strain RD whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EV71 antibody (GTX124261) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wu Y et al. Emerg Microbes Infect 2021; 10 (1) : 1180-1190 A practical method for evaluating the in vivo efficacy of EVA-71 vaccine using a hSCARB2 knock-in mouse model.

Application : IHC-P

Yu P et al. Viruses 2017; 9 (11) Neurotropism In Vitro and Mouse Models of Severe and Mild Infection with Clinical Strains of Enterovirus 71.

Application : IHC-P

Xie GC et al. Virology 2016; 494 : 108-18 Susceptibility of human tonsillar epithelial cells to enterovirus 71 with normal cytokine response.

Application : WB

Xu L et al. Monoclon Antib Immunodiagn Immunother 2013; 32 (6) : 386-94 Monoclonal antibodies for diagnosis of enterovirus 71.

Application : ELISA

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