GeneTex
United States (US)

Enterovirus 71 antibody

GTX124261_40695_WB_18121410_553.jpg
GTX124261_40695_CT_IFA_18121410_573.jpg
GTX124261_40695_20150820_IHC-P_EV71_18121410_292.jpg
GTX124261_40695_20150813_WB_EV71_18121410_872.jpg

Cat No. GTX124261

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Enterovirus 71
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Positive Control

Vero cell lysate infected with EV-71 , EV71 C2 infected RD , EV71 B4 infected RD
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

The immunogen used to generate this antibody corresponds to EV71

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EV71 inactive virus, Enterovirus 71

Research Area

DATA IMAGES
GTX124261_40695_WB_18121410_553.jpg

GTX124261 WB Image

Sample (25 µg of whole cell lysate)
A: Vero cell lysate
B: Vero cell lysate infected with EV-71
10% SDS PAGE
GTX124261 diluted at 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124261_40695_CT_IFA_18121410_573.jpg

GTX124261 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed EV71 infected Vero cell, using EV71 (GTX124261) antibody at 1:1000 and 1:5000 dilution.

GTX124261_40695_20150820_IHC-P_EV71_18121410_292.jpg

GTX124261 IHC-P Image

EV71 antibody detects EV71 viral protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 antibody [GTX124261] diluted at 1:500
Antigen Retrieval: Citrate buffer

GTX124261_40695_20150813_WB_EV71_18121410_872.jpg

GTX124261 WB Image

EV71 antibody detects EV71 protein by western blot analysis. Un-infected (-) and infected with EV71 C2 or B4 strain RD whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EV71 antibody (GTX124261) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169