Enterovirus D68 VP1 antibody [GT11610]

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus D68 VP1, His tag (GTX138561-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus D68 VP1 antibody [HL5333] (GTX645811)
Detection: Enterovirus D68 VP1 antibody [GT11610] (GTX633688)
Pair 2:
Capture: Enterovirus D68 VP1 antibody [HL5334] (GTX645812)
Detection: Enterovirus D68 VP1 antibody [GT11610] (GTX633688)
Pair 3:
Capture: Enterovirus D68 VP1 antibody [HL5433] (GTX646075)
Detection: Enterovirus D68 VP1 antibody [GT11610] (GTX633688)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [GT11610] (GTX633688) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (GTX633688) diluted at 1:4000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below.

Capture: Enterovirus D68 VP1 antibody [GT11610] (GTX633688) (5 μg/mL)
Detection: Enterovirus D68 VP1 antibody [HL1997] (GTX637898) (1 μg/mL)