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Enterovirus D68 VP1 antibody

Orthogonal
Anti-Enterovirus D68 VP1 antibody used in Western Blot (WB). GTX132313
Anti-Enterovirus D68 VP1 antibody used in Western Blot (WB). GTX132313

Cat. No. GTX132313

Reference 25

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Enterovirus D68
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus D68 VP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against Enterovirus D68 VP1, and it does not cross-react with Enterovirus 71 VP1.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus D68

Immunogen

Recombinant protein encompassing a sequence within the center region of enterovirus D68 VP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886609

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Enterovirus D68 Capsid protein VP1 , Enterovirus D68 VP1 , EVD68 Capsid protein VP1 , EVD68 VP1

Research Area

DATA IMAGES

Anti-Enterovirus D68 VP1 antibody used in Western Blot (WB). GTX132313

GTX132313 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody (GTX132313) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus D68 VP1 antibody used in Western Blot (WB). GTX132313

GTX132313 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody (GTX132313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sridhar A et al. Microbiol Spectr 2022; 10 (5) : e0169422 Enterovirus D68 Infection in Human Primary Airway and Brain Organoids: No Additional Role for Heparan Sulfate Binding for Neurotropism.
Li Z et al. J Virol 2022; 96 (2) : e0170821 Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5' Untranslated Region with PCBP1.
Trimarco JD et al. Nat Commun 2022; 13 (1) : 6456 Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection.
Li X et al. J Med Chem 2022; 65 (21) : 14792-14808 Discovery and Optimization of Quinoline Analogues as Novel Potent Antivirals against Enterovirus D68.
Xiaoyan Zhang et al. Exp Ther Med 2022; 23 (3) : 237 Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells.
Application : WB
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REVIEW

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Application Reference
Sridhar A et al. Microbiol Spectr 2022; 10 (5):e0169422 Enterovirus D68 Infection in Human Primary Airway and Brain Organoids: No Additional Role for Heparan Sulfate Binding for Neurotropism.
Li Z et al. J Virol 2022; 96 (2):e0170821 Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5' Untranslated Region with PCBP1.
Trimarco JD et al. Nat Commun 2022; 13 (1):6456 Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection.
Li X et al. J Med Chem 2022; 65 (21):14792-14808 Discovery and Optimization of Quinoline Analogues as Novel Potent Antivirals against Enterovirus D68.
Georgios Stroulios et al. Sci Rep 2022; 12 (1):7673 Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs.
Application : ICC/IF
Xiaoyan Zhang et al. Exp Ther Med 2022; 23 (3):237 Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells.
Application : WB
Yu J et al. Virulence 2022; 13 (1):241-257 Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication.
Application : WB
Si J et al. Virol J 2021; 18 (1):216 High throughput sequencing of whole transcriptome and construct of ceRNA regulatory network in RD cells infected with enterovirus D68.
Application : WB
Raychoudhuri A et al. Virus Res 2021; 304:198528 Development of an experimental inactivated vaccine from Vero cell adapted Enterovirus D68.
Application : WB
Xiao H et al. Virol Sin 2021; (Epub) Ectopic Expression of TRIM25 Restores RIG-I Expression and IFN Production Reduced by Multiple Enteroviruses 3Cpro.
Application : WB
Zhang M et al. Nat Commun 2021; 12 (1):2344 Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases.
Application : ICC/IF
Zhao Z et al. J Virol 2021; 95 (13):e0062021 SAMHD1 Inhibits Multiple Enteroviruses by Interfering with the Interaction between VP1 and VP2 Proteins.
Sooksawasdi Na Ayudhya S et al. mSphere 2020; 5 (6) Enhanced Enterovirus D68 Replication in Neuroblastoma Cells Is Associated with a Cell Culture-Adaptive Amino Acid Substitution in VP1.
Application : WB
Huo W et al. J Microbiol 2020; 58 (9):812-820 Caspase-3 inhibitor inhibits enterovirus D68 production.
Application : WB
Brendan D. Smith et al. bioRxiv 2020; (Epub) Contemporary Enterovirus D68 strains show enhanced replication and translation at 37oC.
Application : FACS
Xu N et al. J Virol 2020; 94 (9) The Pyrimidine Analog FNC Potently Inhibits the Replication of Multiple Enteroviruses.
Application : WB
Musharrafieh R et al. J Med Chem 2019; 62 (8):4074-4090 Discovery of Quinoline Analogues as Potent Antivirals against Enterovirus D68 (EV-D68).
Ma C et al. ACS Infect Dis 2019; 5 (11):1952-1962 A Novel Capsid Binding Inhibitor Displays Potent Antiviral Activity against Enterovirus D68.
Jiang H et al. Elife 2019; 8 Entry by multiple picornaviruses is dependent on a pathway that includes TNK2, WASL, and NCK1.
Guo H et al. Nat Commun 2019; 10 (1):4066 A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells.
Application : WB
Hongbing Jiang et al. bioRxiv 2019; (Epub) Entry by multiple picornaviruses is dependent on a pathway that includes TNK2, WASL and NCK1.
Zhang Y et al. J Immunol 2019; 202 (8):2332-2347 Respiratory Enterovirus (like Parainfluenza Virus) Can Cause Chronic Lung Disease if Protection by Airway Epithelial STAT1 Is Lost.
Application : IHC-P
Musharrafieh R et al. J Virol 2019; (Epub) Validating Enterovirus D68-2Apro as an Antiviral Drug Target and the Discovery of Telaprevir as a Potent D68-2Apro Inhibitor.
Application : WB
David Michael Brown et al. bioRxiv 2018; 2018 (Epub) Contemporary circulating enterovirus D68 strains show differential viral entry and replication in human neuronal cells.
Application : ICC/IF
Kim HS et al. J Biol Chem 2017; (Epub) CRISPR/Cas9-mediated Gene-knockout Screens and Target Identification via Whole Genome Sequencing Uncover Host Genes Required for Picornavirus Infection.
Application : ICC/IF
Reactivity : Enterovirus D68
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169