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Enterovirus D68 VP2 antibody

Anti-Enterovirus D68 VP2 antibody used in Western Blot (WB). GTX132314
Anti-Enterovirus D68 VP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132314

Cat. No. GTX132314

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Enterovirus D68
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus D68 VP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Positive Control

EVD68 infected RD

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus D68

Immunogen

Recombinant protein encompassing a sequence within the center region of enterovirus D68 VP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886610

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus D68 Capsid protein VP2 , Enterovirus D68 VP2 , EVD68 Capsid protein VP2 , EVD68 VP2

Research Area

DATA IMAGES

Anti-Enterovirus D68 VP2 antibody used in Western Blot (WB). GTX132314

GTX132314 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP2 antibody (GTX132314) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Enterovirus D68 VP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132314

GTX132314 IHC-Fr Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28231269

REFERENCE

Application Reference

Raychoudhuri A et al. Virus Res 2021; 304 : 198528 Development of an experimental inactivated vaccine from Vero cell adapted Enterovirus D68.
Application : WB
Evans WJ et al. Antiviral Res 2019; 162 : 61-70 Development of a respiratory disease model for enterovirus D68 in 4-week-old mice for evaluation of antiviral therapies.
Hixon AM et al. J Virol 2019; 93 (16) Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid.
Klaiber N et al. Antiviral Res 2019; 170 : 104565 Susceptibility of Enterovirus-D68 to RNAi-mediated antiviral knockdown.
Application : ICC/IF
Hixon AM et al. PLoS Pathog 2017; 13 (2) : e1006199 A mouse model of paralytic myelitis caused by enterovirus D68.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169