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Envelope protein (JEV) antibody

Anti-Envelope protein (JEV) antibody used in IHC (Paraffin sections) (IHC-P). GTX125867
Anti-Envelope protein (JEV) antibody used in Western Blot (WB). GTX125867
Anti-Envelope protein (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867
Anti-Envelope protein (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

Cat No. GTX125867

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Japanese encephalitis virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Japanese encephalitis virus infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Japanese encephalitis virus (JEV) Envelope protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.

Research Area

DATA IMAGES
Anti-Envelope protein (JEV) antibody used in IHC (Paraffin sections) (IHC-P). GTX125867

GTX125867 IHC-P Image

Envelope protein (JEV) antibody detects Envelope protein (JEV) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded japanese encephalitis virus BHK-21.
Green: Envelope protein (JEV) stained by Envelope protein (JEV) antibody (GTX125867) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Envelope protein (JEV) antibody used in Western Blot (WB). GTX125867

GTX125867 WB Image

envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by western blot analysis.
A. 10 µg BHK-21 whole cell extract
B. 10 µg whole cell extract of Japanese encephalitis virus infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
10% SDS-PAGE
envelope protein (Japanese encephalitis virus) antibody (GTX125867) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Envelope protein (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

GTX125867 ICC/IF Image

Envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in paraformaldehyde.
Green: envelope protein (Japanese encephalitis virus) protein stained by Envelope protein (Japanese encephalitis virus) antibody (GTX125867) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Envelope protein (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

GTX125867 ICC/IF Image

Envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: envelope protein (Japanese encephalitis virus) protein stained by Envelope protein (Japanese encephalitis virus) antibody (GTX125867) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

Envelope protein (JEV) antibody Cat No. GTX125867

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-Aug-2014
Score :
Application Tested : IHC-P
Sample Species : JEV
Sample : Japanese encephalitis virus infected mouse brain
Blocking : Normal Goat Serum Blocking in TBS, 25°C, 0.3Hr
Fixation : 10% Formalin
Permeabilization /Antigen retrieval : TE buffer
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf