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EpCAM antibody [EGP40/1120]

Anti-EpCAM antibody [EGP40/1120] used in Western Blot (WB). GTX34692
Anti-EpCAM antibody [EGP40/1120] used in IHC (Paraffin sections) (IHC-P). GTX34692

Cat No. GTX34692

Host Mouse
Clonality Monoclonal
Clone Name EGP40/1120
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.25-0.5ug/ml for 30 min at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant full-length human EpCAM protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epithelial Cell Adhesion Molecule , Diar5 , Egp-2 , Egp314 , Egp40 , Esa , Hnpcc8 , Ks1/4 , Ksa , M4S1 , Mic18 , Mk-1 , Tacstd1 , Trop1 , Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES
Anti-EpCAM antibody [EGP40/1120] used in Western Blot (WB). GTX34692

GTX34692 WB Image

Western Blot Analysis of HCT116 Cell Lysate using EpCAM Monoclonal Antibody (EGP40/1120)

Anti-EpCAM antibody [EGP40/1120] used in IHC (Paraffin sections) (IHC-P). GTX34692

GTX34692 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/1120).

REFERENCE
There are currently no references for EpCAM antibody [EGP40/1120] (GTX34692). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 429