EpCAM antibody [EGP40/1372]

Cat. No. GTX34694

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	EGP40/1372
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, Protein Array
Reactivity	Human
Package	100 μg ($529)

See all EpCAM products

View product citations for antibody GTX34694 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 min at RT
FACS	1-2μg/10⁶ cells
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human EpCAM fragment from extracellular domain (around aa 77-202) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Gene ID: 4072 EPCAM
UniProt: P16422 EPCAM

Research Area

DATA IMAGES

GTX34694 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

GTX34694 IHC-P Image

IHC-P analysis of human colorectal carcinoma tissue using GTX34694 EpCAM antibody [EGP40/1372].

GTX34694 WB Image

WB analysis of 293, A431 and, HCT116 cell lysates using GTX34694 EpCAM antibody [EGP40/1372].

GTX34694 IHC-P Image

IHC-P analysis of human colorectal carcinoma tissue using GTX34694 EpCAM antibody [EGP40/1372].

GTX34694 WB Image

WB analysis of human lung tissue lysate using GTX34694 EpCAM antibody [EGP40/1372].

GTX34694 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34694 EpCAM antibody [EGP40/1372].
Green : Primary antibody
Red : nucleus

GTX34694 IHC-P Image

IHC-P analysis of human hepatocellular carcinoma tissue using GTX34694 EpCAM antibody [EGP40/1372].

GTX34694 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX34694 EpCAM antibody [EGP40/1372].
Blue : Primary antibody
Red : Isotype control

REFERENCE

Application Reference

Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells

Application : ICC/IF

Submit a Reference

REVIEW

There are currently no reviews for EpCAM antibody [EGP40/1372] (GTX34694). Be the first to share your experience with this product.

Submit a Review

Application Reference

Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells

Application : ICC/IF

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty

EpCAM antibody [EGP40/1372]

Cat. No. GTX34694

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX34694 Protein Array Image

GTX34694 IHC-P Image

GTX34694 WB Image

GTX34694 IHC-P Image

GTX34694 WB Image

GTX34694 ICC/IF Image

GTX34694 IHC-P Image

GTX34694 FACS Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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