EpCAM antibody [EGP40/1373]

Cat No. GTX34695

Host	Mouse
Clonality	Monoclonal
Clone Name	EGP40/1373
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS, Protein Array
Reactivity	Human
Package	100 μg ($339)

See all EpCAM products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2μg/ml
ICC/IF	1-3μg/ml
IHC-P	1-2μg/ml for 30 min at RT
FACS	1-2μg/10⁶ cells
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Gene ID: 4072 EPCAM
UniProt: P16422 EPCAM

Research Area

DATA IMAGES

GTX34695 IHC-P Image

IHC-P analysis of human fallopiantube tissue using GTX34695 EpCAM antibody [EGP40/1373].

GTX34695 WB Image

WB analysis of human lung tissue lysate using GTX34695 EpCAM antibody [EGP40/1373].

GTX34695 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34695 EpCAM antibody [EGP40/1373].
Green : Primary antibody
Red : nucleus

GTX34695 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1373). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 339

Qty

EpCAM antibody [EGP40/1373]

Cat No. GTX34695

Host

Clonality

Clone Name

Isotype

Application

Reactivity