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EpCAM antibody [EGP40/826]

Anti-EpCAM antibody [EGP40/826] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34704
Anti-EpCAM antibody [EGP40/826] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34704
Anti-EpCAM antibody [EGP40/826] used in IHC (Paraffin sections) (IHC-P). GTX34704

Cat No. GTX34704

Host Mouse
Clonality Monoclonal
Clone Name EGP40/826
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epithelial Cell Adhesion Molecule,Diar5,Egp-2,Egp314,Egp40,Esa,Hnpcc8,Ks1/4,Ksa,M4S1,Mic18,Mk-1,Tacstd1,Trop1,Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES
Anti-EpCAM antibody [EGP40/826] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34704

GTX34704 ICC/IF Image

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Monoclonal Antibody (EGP40/826) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Anti-EpCAM antibody [EGP40/826] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34704

GTX34704 ICC/IF Image

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG1) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Anti-EpCAM antibody [EGP40/826] used in IHC (Paraffin sections) (IHC-P). GTX34704

GTX34704 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/826).

Package List Price ($)
$ 329