*Optimal dilutions/concentrations should be determined by the researcher.
|0.5-1ug/ml for 30 minutes at RT
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein
Ab purified from Bioreactor Concentrate by Protein A/G
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Epithelial Cell Adhesion Molecule,Diar5,Egp-2,Egp314,Egp40,Esa,Hnpcc8,Ks1/4,Ksa,M4S1,Mic18,Mk-1,Tacstd1,Trop1,Epcam
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
GTX34704 ICC/IF Image
Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Monoclonal Antibody (EGP40/826) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).
GTX34704 ICC/IF Image
Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG1) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)
GTX34704 IHC-P Image
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/826).