EpCAM antibody [EGP40/837]

Cat No. GTX34705

Host	Mouse
Clonality	Monoclonal
Clone Name	EGP40/837
Isotype	IgG1
Application	IHC-P, FACS
Reactivity	Human

See all EpCAM products

Datasheet

APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 min at RT, FACS:0.5-1ug/million cells in 0.1ml.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human TACSTD1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Epithelial Cell Adhesion Molecule,Diar5,Egp-2,Egp314,Egp40,Esa,Hnpcc8,Ks1/4,Ksa,M4S1,Mic18,Mk-1,Tacstd1,Trop1,Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]