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EpCAM antibody [EGP40/837]

Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705
Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705
Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705
Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705
Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705
Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705
Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

Cat No. GTX34705

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EGP40/837

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($469)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TACSTD1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705

GTX34705 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34705 EpCAM antibody [EGP40/837].
Green : Primary antibody
Red : nucleus

Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705

GTX34705 FACS Image

FACS analysis of MCF-7 cells using GTX34705 EpCAM antibody [EGP40/837].
Black : Blank
Green : Isotype Control
Red : Primary antibody

Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705

GTX34705 ICC/IF Image

ICC/IF analysis of SK-OV-3 cells using GTX34705 EpCAM antibody [EGP40/837].
Green : Primary antibody
Blue : nucleus

Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

GTX34705 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34705 EpCAM antibody [EGP40/837].

Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705

GTX34705 WB Image

WB analysis of HCT116 cell lysate using GTX34705 EpCAM antibody [EGP40/837].

Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

GTX34705 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34705 EpCAM antibody [EGP40/837].

Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

GTX34705 IHC-P Image

IHC-P analysis of human endometrial cancer tissue using GTX34705 EpCAM antibody [EGP40/837].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469