APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 min at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
We do not recommend use of this product for Mouse,Rat.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human TACSTD1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326
Cellular Localization
Lateral cell membrane,Cell junction,Tight junction
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Research Area
DATA IMAGES
![Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_WB_90_20011518_594.jpg "Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705")
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GTX34705 WB Image
WB analysis of HCT116 cell lysate using GTX34705 EpCAM antibody [EGP40/837].
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![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_ICC-IF_1572_20011518_304.jpg "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
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GTX34705 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX34705 EpCAM antibody [EGP40/837].
Green : Primary antibody
Red : nucleus
|
![Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_FACS_1549_20011518_877.jpg "Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705")
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GTX34705 FACS Image
FACS analysis of MCF-7 cells using GTX34705 EpCAM antibody [EGP40/837].
Black : Blank
Green : Isotype Control
Red : Primary antibody
|
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_ICC-IF_1872_20011518_852.jpg "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
|
GTX34705 ICC/IF Image
ICC/IF analysis of SK-OV-3 cells using GTX34705 EpCAM antibody [EGP40/837].
Green : Primary antibody
Blue : nucleus
|
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_IHC-P_467_20011518_158.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
|
GTX34705 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34705 EpCAM antibody [EGP40/837].
|
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_IHC-P_529_20011518_558.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
|
GTX34705 IHC-P Image
IHC-P analysis of human endometrial cancer tissue using GTX34705 EpCAM antibody [EGP40/837].
|
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/middle/GTX34705/GTX34705_20200115_IHC-P_818_20011518_995.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
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GTX34705 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX34705 EpCAM antibody [EGP40/837].
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REFERENCE
There are currently no references for EpCAM antibody [EGP40/837] (GTX34705). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EpCAM antibody [EGP40/837] (GTX34705). Be the first to share your experience with this product.
![Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_WB_90_20011518_594.jpg "Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_ICC-IF_1572_20011518_304.jpg "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_FACS_1549_20011518_877.jpg "Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_ICC-IF_1872_20011518_852.jpg "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_IHC-P_467_20011518_158.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_IHC-P_529_20011518_558.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_IHC-P_818_20011518_995.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_WB_90_20011518_594.jpg "Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_ICC-IF_1572_20011518_304.jpg "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_FACS_1549_20011518_877.jpg "Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_ICC-IF_1872_20011518_852.jpg "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_IHC-P_467_20011518_158.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_IHC-P_529_20011518_558.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_IHC-P_818_20011518_995.jpg "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
