APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 min at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Mouse,Rat samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human TACSTD1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326
Cellular Localization
Lateral cell membrane,Cell junction,Tight junction
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Research Area
DATA IMAGES
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/templates/default/js/lightbox/images/loading.gif "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
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GTX34705 ICC/IF Image
ICC/IF analysis of SK-OV-3 cells using GTX34705 EpCAM antibody [EGP40/837].
Green : Primary antibody
Blue : nucleus
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GTX34705 FACS Image
FACS analysis of MCF-7 cells using GTX34705 EpCAM antibody [EGP40/837].
Black : Blank
Green : Isotype Control
Red : Primary antibody
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GTX34705 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34705 EpCAM antibody [EGP40/837].
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GTX34705 IHC-P Image
IHC-P analysis of human endometrial cancer tissue using GTX34705 EpCAM antibody [EGP40/837].
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GTX34705 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX34705 EpCAM antibody [EGP40/837].
Green : Primary antibody
Red : nucleus
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GTX34705 WB Image
WB analysis of HCT116 cell lysate using GTX34705 EpCAM antibody [EGP40/837].
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GTX34705 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX34705 EpCAM antibody [EGP40/837].
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REFERENCE
There are currently no references for EpCAM antibody [EGP40/837] (GTX34705). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EpCAM antibody [EGP40/837] (GTX34705). Be the first to share your experience with this product.
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_ICC-IF_1872_w_23060801_307.webp "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_FACS_1549_w_23060801_521.webp "Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_IHC-P_467_w_23060801_995.webp "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_IHC-P_529_w_23060801_452.webp "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_ICC-IF_1572_w_23060801_528.webp "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_WB_90_w_23060801_396.webp "Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/small/GTX34705/GTX34705_20200115_IHC-P_818_w_23060801_272.webp "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_ICC-IF_1872_w_23060801_307.webp "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_FACS_1549_w_23060801_521.webp "Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_IHC-P_467_w_23060801_995.webp "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_IHC-P_529_w_23060801_452.webp "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_ICC-IF_1572_w_23060801_528.webp "Anti-EpCAM antibody [EGP40/837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_WB_90_w_23060801_396.webp "Anti-EpCAM antibody [EGP40/837] used in Western Blot (WB). GTX34705")
![Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705](/upload/website/prouct_img/normal/GTX34705/GTX34705_20200115_IHC-P_818_w_23060801_272.webp "Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705")