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EpCAM antibody [EGP40/837]

Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705
Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705
Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705
Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

Cat No. GTX34705

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EGP40/837

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($429)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human TACSTD1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Epithelial Cell Adhesion Molecule , Diar5 , Egp-2 , Egp314 , Egp40 , Esa , Hnpcc8 , Ks1/4 , Ksa , M4S1 , Mic18 , Mk-1 , Tacstd1 , Trop1 , Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [EGP40/837] used in Flow cytometry (FACS). GTX34705

GTX34705 FACS Image

Flow Cytometry of human MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Ep-CAM Monoclonal Antibody (EGP40/837).

Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

GTX34705 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/837).

Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

GTX34705 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/837).

Anti-EpCAM antibody [EGP40/837] used in IHC (Paraffin sections) (IHC-P). GTX34705

GTX34705 IHC-P Image

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/837).

REFERENCE

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REVIEW

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Package List Price ($)
$ 429