GeneTex
United States (US)

EpCAM antibody [ESA 214] (FITC)

Cat No. GTX30708

Host Mouse
Clonality Monoclonal
Clone Name ESA 214
Isotype IgG1
Application ICC/IF, FACS, IHC
Reactivity Human
APPLICATION

Application Note

IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. IF, FITC~conjugated antibodies 10-20 μg/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. FITC~conjugated antibodies, 0.2-1.0 μg/0.1 ml (1:200-1:1,000)

Calculated MW

35 kDa. ( Note )

Positive Control

Breast carcinoma
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4 containing 1% BSA and 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.20 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma

Purification

Protein A purified

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epithelial Cell Adhesion Molecule,Diar5,Egp-2,Egp314,Egp40,Esa,Hnpcc8,Ks1/4,Ksa,M4S1,Mic18,Mk-1,Tacstd1,Trop1,Epcam

Cellular Localization

Cell membrane

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

Package List Price ($)
$ 319