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EpCAM antibody [ESA 214] (FITC)

Cat. No. GTX30708

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 ESA 214

Isotype

 IgG1

Application

 ICC/IF, FACS, IHC

Reactivity

 Human
Package
500 μl ($399)
See all EpCAM products

APPLICATION

Application Note

IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. IF, FITC~conjugated antibodies 10-20 μg/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. FITC~conjugated antibodies, 0.2-1.0 μg/0.1 ml (1:200-1:1,000)

Calculated MW

35 kDa. ( Note )

Positive Control

Breast carcinoma

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.20 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma

Purification

Protein A purified

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

REFERENCE

Application Reference

Xu T et al. Cell Death Differ 2024; 31 (3) : 360-377 Tropomyosin1 isoforms underlie epithelial to mesenchymal plasticity, metastatic dissemination, and resistance to chemotherapy in high-grade serous ovarian cancer.
Strelez C et al. bioRxiv 2023; Integration of Patient-Derived Organoids and Organ-on-Chip Systems: Investigating Colorectal Cancer Invasion within the Mechanical and GABAergic Tumor Microenvironment.
Windster JD et al. EMBO Rep 2023; 24 (4) : e55789 A combinatorial panel for flow cytometry-based isolation of enteric nervous system cells from human intestine.
Application : FACS
Reactivity : Human
Xu T et al. Elife 2022; 11 Alternative splicing downstream of EMT enhances phenotypic plasticity and malignant behavior in colon cancer.
Application : FACS
Reactivity : Human
Andrea Sacchetti et al. Elife 2021; 10 Phenotypic plasticity underlies local invasion and distant metastasis in colon cancer.
Application : FACS
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399