EpCAM antibody [GT25512]

Cat. No. GTX635967

Host	Mouse
Clonality	Monoclonal
Clone Name	GT25512
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX635967 on CiteAb

PRODUCT

Summary

EpCAM antibody recognizes epithelial cell adhesion molecule (EpCAM), a transmembrane glycoprotein with a predicted molecular weight of ~35 kDa. EpCAM is upregulated in epithelial cells during epithelial–mesenchymal transition (EMT) and is used as a diagnostic marker of many carcinomas and circulating tumor cells. Detection of EpCAM by immunohistochemistry is important for the diagnosis of certain epithelial malignancies.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Negative Control

HeLa

Product Note

This antibody was raised against human EpCAM Extracellular domain.
We do not recommend use of this product for Mouse samples.

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human EpCAM. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909924

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Gene ID: 4072 EPCAM
UniProt: P16422 EPCAM

Research Area

DATA IMAGES

GTX635967 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with EpCAM antibody [GT25512] (GTX635967) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX2131

GTX635967 IHC-P Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231 xenograft.
EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635967 ICC/IF Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:500.

GTX635967 FACS Image

EpCAM antibody[GT25512] (GTX635967) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT25512] (GTX635967) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)
HCT116 (EpCAM positive), HeLa (EpCAM negative)

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty