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EpCAM antibody [GT25512]

Anti-EpCAM antibody [GT25512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635967
Anti-EpCAM antibody [GT25512] used in IHC (Paraffin sections) (IHC-P). GTX635967
Anti-EpCAM antibody [GT25512] used in Flow cytometry (FACS). GTX635967
Anti-EpCAM antibody [GT25512] used in Western Blot (WB). GTX635967

Cat No. GTX635967

Host

Mouse

Clonality

Monoclonal

Clone Name

GT25512

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)
PRODUCT

Summary

EpCAM antibody recognizes epithelial cell adhesion molecule (EpCAM), a transmembrane glycoprotein with a predicted molecular weight of ~35 kDa. EpCAM is upregulated in epithelial cells during epithelial–mesenchymal transition (EMT) and is used as a diagnostic marker of many carcinomas and circulating tumor cells. Detection of EpCAM by immunohistochemistry is important for the diagnosis of certain epithelial malignancies.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Negative Control

HeLa

Product Note

We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909924

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [GT25512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635967

GTX635967 ICC/IF Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:500.

Anti-EpCAM antibody [GT25512] used in IHC (Paraffin sections) (IHC-P). GTX635967

GTX635967 IHC-P Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231 xenograft.
EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [GT25512] used in Flow cytometry (FACS). GTX635967

GTX635967 FACS Image

EpCAM antibody[GT25512] (GTX635967) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT25512] (GTX635967) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)
HCT116 (EpCAM positive), HeLa (EpCAM negative)

Anti-EpCAM antibody [GT25512] used in Western Blot (WB). GTX635967

GTX635967 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with EpCAM antibody [GT25512] (GTX635967) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX2131

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169