*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
We do not recommend use of this product for Mouse samples.
Mouse, Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PBS, 20% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
GTX635970 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EpCAM antibody[GT3188] (GTX635970) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX635970 ICC/IF Image
EpCAM antibody [GT3188] detects EpCAM protein at cell membrane by immunofluorescent analysis.Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.Green: EpCAM stained by EpCAM antibody [GT3188] (GTX635970) diluted at 1:500.
GTX635970 FACS Image
EpCAM antibody[GT3188] (GTX635970) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT3188] (GTX635970) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)
GTX635970 IHC-P Image
EpCAM antibody [GT3188] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded MDA-MB-231 xenograft.EpCAM stained by EpCAM antibody [GT3188] (GTX635970) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min