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EpCAM antibody [GT852]

Anti-EpCAM antibody [GT852] used in Western Blot (WB). GTX635474
Anti-EpCAM antibody [GT852] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635474

Cat No. GTX635474

Host

Mouse

Clonality

Monoclonal

Clone Name

GT852

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888533

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [GT852] used in Western Blot (WB). GTX635474

GTX635474 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EpCAM antibody [GT852] (GTX635474) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EpCAM antibody [GT852] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635474

GTX635474 ICC/IF Image

EpCAM antibody [GT852] detects EpCAM protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [GT852] (GTX635474) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for EpCAM antibody [GT852] (GTX635474). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EpCAM antibody [GT852] (GTX635474). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 379
$ 169