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EpCAM antibody [HEA125]

Cat No. GTX76570

Host

Mouse

Clonality

Monoclonal

Clone Name

HEA125

Isotype

IgG1

Application

ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human
Package
100 μg ($899)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P 1/20
IHC-Fr 1/20
FACS Neat

Note :

IHC-P
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

FACS
Use 5μl of the suggested working dilution to label 1006 cells or 100μl celll suspension.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide, 0.5% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HT29 carcinoma cell line.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Epithelial Cell Adhesion Molecule , Diar5 , Egp-2 , Egp314 , Egp40 , Esa , Hnpcc8 , Ks1/4 , Ksa , M4S1 , Mic18 , Mk-1 , Tacstd1 , Trop1 , Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 899