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EpCAM antibody [HL1339]

Recombinant antibody Orthogonal
Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759
Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759
Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

Cat. No. GTX636759

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1339

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910068

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759

GTX636759 FACS Image

Orthogonal

EpCAM antibody [HL1339] (GTX636759) detects EpCAM protein by flow cytometry analysis.
Sample: HCT-116 and HeLa cell.
Gray: Rabbit IgG Isotype control.
Red: EpCAM antibody [HL1339] (GTX636759) dilution: 1:50.
Acquisition of 50,000 events were collected for flow cytometry analysis.

Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759

GTX636759 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1339] (GTX636759) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human HCT116 xenograft.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

GTX636759 ICC/IF Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

GTX636759 ICC/IF Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

P Bisaccia et al. Stem Cells Transl Med 2023; : szad070 Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia
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Package List Price ($)
$ 399
$ 169
Custom Conjugations