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EpCAM antibody [HL1339]

Recombinant antibody Orthogonal
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759
Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759
Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759
Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

Cat No. GTX636759

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1339

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910068

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759

GTX636759 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1339] (GTX636759) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759

GTX636759 FACS Image

Orthogonal

EpCAM antibody [HL1339] (GTX636759) detects EpCAM protein by flow cytometry analysis.
Sample: HCT-116 and HeLa cell.
Gray: Rabbit IgG Isotype control.
Red: EpCAM antibody [HL1339] (GTX636759) dilution: 1:50.
Acquisition of 50,000 events were collected for flow cytometry analysis.

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759

GTX636759 IHC-P Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human HCT116 xenograft.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

GTX636759 ICC/IF Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759

GTX636759 ICC/IF Image

EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations