APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910068
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Research Area
DATA IMAGES
![Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759](/templates/default/js/lightbox/images/loading.gif "Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759")
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GTX636759 FACS Image
EpCAM antibody [HL1339] (GTX636759) detects EpCAM protein by flow cytometry analysis.
Sample: HCT-116 and HeLa cell.
Gray: Rabbit IgG Isotype control.
Red: EpCAM antibody [HL1339] (GTX636759) dilution: 1:50.
Acquisition of 50,000 events were collected for flow cytometry analysis.
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GTX636759 IHC-P Image
EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636759 IHC-P Image
EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636759 IHC-P Image
EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636759 IHC-P Image
EpCAM antibody [HL1339] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human HCT116 xenograft.
EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636759 ICC/IF Image
EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX636759 ICC/IF Image
EpCAM antibody [HL1339] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1339] (GTX636759) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX636759 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1339] (GTX636759) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for EpCAM antibody [HL1339] (GTX636759). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EpCAM antibody [HL1339] (GTX636759). Be the first to share your experience with this product.
![Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220829_FACS_22090701_123.webp "Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220422_IHC-P_M_22050323_858.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220422_IHC-P_R_22050323_137.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220527_IHC-P_M_22060523_239.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220527_IHC-P_22060523_329.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_T-44578_20220624_ICC_IF_22062919_741.webp "Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220624_ICC_IF_22062919_484.webp "Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759](/upload/website/prouct_img/small/GTX636759/GTX636759_44634_20220401_WB_22041219_852.jpg "Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220829_FACS_22090701_123.webp "Anti-EpCAM antibody [HL1339] used in Flow cytometry (FACS). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220422_IHC-P_M_22050323_858.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220422_IHC-P_R_22050323_137.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220527_IHC-P_M_22060523_239.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220527_IHC-P_22060523_329.jpg "Anti-EpCAM antibody [HL1339] used in IHC (Paraffin sections) (IHC-P). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_T-44578_20220624_ICC_IF_22062919_741.webp "Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220624_ICC_IF_22062919_484.webp "Anti-EpCAM antibody [HL1339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636759")
![Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759](/upload/website/prouct_img/normal/GTX636759/GTX636759_44634_20220401_WB_22041219_852.jpg "Anti-EpCAM antibody [HL1339] used in Western Blot (WB). GTX636759")