EpCAM antibody [HL1524]

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1524] (GTX636998) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

EpCAM antibody [HL1524] (GTX636998) detects EpCAM protein by flow cytometry analysis.
Sample: HCT-116, HeLa and 4T1 cell.
Gray: Rabbit IgG Isotype control.
Red: EpCAM antibody [HL1524] (GTX636998) dilution: 1:50.
Acquisition of 50,000 (HCT-116 and HeLa cell) and 100,000 (4T1 cell) events were collected for flow cytometry analysis.

EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1524] (GTX636998) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EpCAM antibody [HL1524] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded HCT116 xenograft.
EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min