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EpCAM antibody [N3C3]

Anti-EpCAM antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113091
Anti-EpCAM antibody [N3C3] used in Western Blot (WB). GTX113091
Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091
Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091
Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091

Cat. No. GTX113091

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950208

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113091

GTX113091 ICC/IF Image

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EpCAM antibody [N3C3] used in Western Blot (WB). GTX113091

GTX113091 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [N3C3] (GTX113091) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091

GTX113091 IHC-P Image

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091

GTX113091 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using Ep-CAM (GTX113091) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091

GTX113091 IHC-P Image

The data was published in the journal Oncotarget in 2016. PMID: 27564110

REFERENCE

Application Reference

Zhang K et al. Mol Cancer 2018; 17 (1) : 153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Lee YH et al. Oncotarget 2016; 7 (40) : 65589-65601 Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
Application : IHC-P
Reactivity : Human
Al-Lamki RS et al. Oncotarget 2016; 7 (17) : 24111-24 Tumor necrosis factor receptor 2-signaling in CD133-expressing cells in renal clear cell carcinoma.
Reactivity : Human
Sridaran D et al. Tumour Biol 2016; Oxystressed tumor microenvironment potentiates epithelial to mesenchymal transition and alters cellular bioenergetics towards cancer progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169