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EpCAM antibody [N3C3]

Anti-EpCAM antibody [N3C3] used in Western Blot (WB). GTX113091
Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091
Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091
Anti-EpCAM antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113091

Cat No. GTX113091

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

H1299 , HCT116 , MCF-7 , Molt-4

Predict Reactivity

Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950208

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [N3C3] used in Western Blot (WB). GTX113091

GTX113091 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [N3C3] (GTX113091) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091

GTX113091 IHC-P Image

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EpCAM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113091

GTX113091 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using Ep-CAM (GTX113091) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EpCAM antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113091

GTX113091 ICC/IF Image

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

EpCAM antibody [N3C3] Cat No. GTX113091

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27564110
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169