GeneTex
United States (US)

EpCAM antibody [N3C3]

GTX113091_40800_20160121_WB_18121410_412.jpg
GTX113091_40114_20171208_IHC-P_M_18121410_742.jpg
GTX113091_IHC_18121410_100.jpg
GTX113091_40114_WB_2_18121410_557.jpg

Cat No. GTX113091

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

H1299 , HCT116 , MCF-7 , Molt-4

Predict Reactivity

Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, GA733-2 antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, EPCAM antibody, major gastrointestinal tumor-associated protein GA733-2 antibody, epithelial glycoprotein 314 antibody, human epithelial glycoprotein-2 antibody, tumor-associated calcium signal transducer 1 antibody, "membrane component, chromosome 4, surface marker (35kD glycoprotein) antibody", adenocarcinoma-associated antigen antibody, epithelial cell adhesion molecule antibody, cell surface glycoprotein Trop-1 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113091_40800_20160121_WB_18121410_412.jpg

GTX113091 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EpCAM antibody [N3C3] (GTX113091) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113091_40114_20171208_IHC-P_M_18121410_742.jpg

GTX113091 IHC-P Image

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113091_IHC_18121410_100.jpg

GTX113091 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using Ep-CAM (GTX113091) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113091_40114_WB_2_18121410_557.jpg

GTX113091 WB Image

EpCAM antibody [N3C3] detects EpCAM protein by western blot analysis.
A. 30 µg Molt-4 whole cell extract
12% SDS-PAGE
EpCAM antibody [N3C3] (GTX113091) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169