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EpCAM antibody [PAN-EpCAM (Cocktail)]

Anti-EpCAM antibody [PAN-EpCAM (Cocktail)] used in IHC (Paraffin sections) (IHC-P). GTX34706

Cat No. GTX34706

Host

Mouse

Clonality

Monoclonal

Clone Name

PAN-EpCAM (Cocktail)

Isotype

Mixed clones

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

It is a cocktail of four highly specific monoclonal antibodies (EGP40/826, EGP40/837, EGP40/1110, EGP40/1120) that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or Ep-CAM. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human Ep-CAM protein (full-length and fragments)

Purification

Abs purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Epithelial Cell Adhesion Molecule , Diar5 , Egp-2 , Egp314 , Egp40 , Esa , Hnpcc8 , Ks1/4 , Ksa , M4S1 , Mic18 , Mk-1 , Tacstd1 , Trop1 , Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [PAN-EpCAM (Cocktail)] used in IHC (Paraffin sections) (IHC-P). GTX34706

GTX34706 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (PAN-EpCAM).

REFERENCE

There are currently no references for EpCAM antibody [PAN-EpCAM (Cocktail)] (GTX34706). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 429