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EpCAM antibody [SPM134]

Anti-EpCAM antibody [SPM134] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34699
Anti-EpCAM antibody [SPM134] used in IHC (Paraffin sections) (IHC-P). GTX34699

Cat No. GTX34699

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM134

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT

Note :

IHC-P
Staining of formalin/paraffin tissues require digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37ºC.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

We do not recommend use of this product for Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [SPM134] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34699

GTX34699 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34699 EpCAM antibody [SPM134].
Green : Primary antibody
Red : nucleus

Anti-EpCAM antibody [SPM134] used in IHC (Paraffin sections) (IHC-P). GTX34699

GTX34699 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34699 EpCAM antibody [SPM134].

REFERENCE

There are currently no references for EpCAM antibody [SPM134] (GTX34699). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EpCAM antibody [SPM134] (GTX34699). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339