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EpCAM antibody [SPM134]

Anti-EpCAM antibody [SPM134] used in IHC (Paraffin sections) (IHC-P). GTX34699
Anti-EpCAM antibody [SPM134] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34699

Cat. No. GTX34699

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM134

Isotype

 IgG1

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT

Note :

IHC-P
Staining of formalin/paraffin tissues require digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37ºC.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [SPM134] used in IHC (Paraffin sections) (IHC-P). GTX34699

GTX34699 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34699 EpCAM antibody [SPM134].

Anti-EpCAM antibody [SPM134] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34699

GTX34699 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34699 EpCAM antibody [SPM134].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529