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EpCAM antibody [VU-1D9] (Low endotoxin, azide free)

Anti-EpCAM antibody [VU-1D9] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42071

Cat. No. GTX42071

Reference 8

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VU-1D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human
Package
500 μg ($739)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/10-1/200
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HG9 cell line (small cell lung carcinoma)

Purification

Protein G purified

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [VU-1D9] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42071

GTX42071 FACS Image

FACS analysis of HT-29 cells using GTX42071 EpCAM antibody [VU-1D9] (Low endotoxin, azide free).

REFERENCE

Clone Reference

Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Application : FACS
Reactivity : Human
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 739
Custom Conjugations