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EpCAM antibody [VU-1D9] (PE-Cy7)

Cat. No. GTX00606-10

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VU-1D9

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($319)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

This antibody recognizes an epitope within EGF-like domain I of CD326 / EpCAM.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma cell line H69.

Purification

Purified by size-exclusion chromatography

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Cy and CyDye are trademarks of GE Healthcare.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

REFERENCE

Clone Reference

Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319