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EpCAM antibody [VU-1D9]

Anti-EpCAM antibody [VU-1D9] used in IHC (Paraffin sections) (IHC-P). GTX28667

Cat. No. GTX28667

Reference 10

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VU-1D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
250 μl ($319)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1-2 μg/ml
FACS Assay dependent

Note :

IHC-P
Digestion of tissue sections with pepsin at 1 mg/ml Tris-HCl (pH 2.0) for 15 min at RT or 10 min at 37ºC.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS (pH7.4), 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma cells

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368463

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [VU-1D9] used in IHC (Paraffin sections) (IHC-P). GTX28667

GTX28667 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX28667 EpCAM antibody [VU-1D9].

REFERENCE

Clone Reference

Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Application : FACS
Reactivity : Human
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
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REVIEW

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Application Reference
Chen HH et al. J Biomed Opt 2014; 19 (4):45002 High-purity separation of cancer cells by optically induced dielectrophoresis.
Clone Reference
Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Application : FACS
Reactivity : Human
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
Stephen Kershaw et al. BMC Cancer 2015; 15:387 Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells
Application : WB
Reactivity : Human
Ma R et al. Lab Invest 2014; 94 (7):796-805 Superficial scrapings from breast tumors is a source for biobanking and research purposes.
Application : ICC/IF
Reactivity : Human
Okuda H et al. Cancer Res 2012; 72 (2):537-47 Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells with macrophages and stromal cells.
Kimura O et al. Cancer Sci 2010; 101 (10):2145-55 Characterization of the epithelial cell adhesion molecule (EpCAM)+ cell population in hepatocellular carcinoma cell lines.
Package List Price ($)
$ 319