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EpCAM antibody [VU-1D9]

Anti-EpCAM antibody [VU-1D9] used in Flow cytometry (FACS). GTX34709
Anti-EpCAM antibody [VU-1D9] used in IHC (Paraffin sections) (IHC-P). GTX34709
Anti-EpCAM antibody [VU-1D9] used in Flow cytometry (FACS). GTX34709
Anti-EpCAM antibody [VU-1D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34709

Cat No. GTX34709

Host

Mouse

Clonality

Monoclonal

Clone Name

VU-1D9

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM.
This product does not react with Rat,Ferret.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Small cell lung carcinoma cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DIAR5,EGP2,EGP314,EGP40,EPCAM,ESA,HNPCC8,KS1/4,KSA,M4S1,MIC18,MK1,TACSTD1,TROP1,epithelial cell adhesion molecule,EpCAM,CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [VU-1D9] used in Flow cytometry (FACS). GTX34709

GTX34709 FACS Image

FACS analysis of HT29 cells using GTX34709 EpCAM antibody [VU-1D9].
Red : Primary antibody
Green : Isotype control

Anti-EpCAM antibody [VU-1D9] used in IHC (Paraffin sections) (IHC-P). GTX34709

GTX34709 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34709 EpCAM antibody [VU-1D9].

Anti-EpCAM antibody [VU-1D9] used in Flow cytometry (FACS). GTX34709

GTX34709 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX34709 EpCAM antibody [VU-1D9].
Blue : Primary antibody
Red : Isotype control

Anti-EpCAM antibody [VU-1D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34709

GTX34709 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34709 EpCAM antibody [VU-1D9].
Green : Primary antibody
Red : nucleus

REFERENCE

There are currently no references for EpCAM antibody [VU-1D9] (GTX34709). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EpCAM antibody [VU-1D9] (GTX34709). Be the first to share your experience with this product.
Package List Price ($)
$ 339