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EpCAM antibody [rMOC-31]

Anti-EpCAM antibody [rMOC-31] used in IHC (Paraffin sections) (IHC-P). GTX34698
Anti-EpCAM antibody [rMOC-31] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34698

Cat No. GTX34698

Host

Mouse

Clonality

Monoclonal

Clone Name

rMOC-31

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of EpCAM.
This product does not react with Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant full-length human EpCAM protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DIAR5,EGP2,EGP314,EGP40,EPCAM,ESA,HNPCC8,KS1/4,KSA,M4S1,MIC18,MK1,TACSTD1,TROP1,epithelial cell adhesion molecule,EpCAM,CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EpCAM antibody [rMOC-31] used in IHC (Paraffin sections) (IHC-P). GTX34698

GTX34698 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34698 EpCAM antibody [rMOC-31].

Anti-EpCAM antibody [rMOC-31] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34698

GTX34698 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34698 EpCAM antibody [rMOC-31].
Green : Primary antibody
Red : nucleus

REFERENCE

There are currently no references for EpCAM antibody [rMOC-31] (GTX34698). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EpCAM antibody [rMOC-31] (GTX34698). Be the first to share your experience with this product.
Package List Price ($)
$ 339