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EphA1 antibody [C1C3-2]

Anti-EphA1 antibody [C1C3-2] used in Western Blot (WB). GTX111449
Anti-EphA1 antibody [C1C3-2] used in Western Blot (WB). GTX111449

Cat. No. GTX111449

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all EphA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Eph Receptor A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722296

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor A1 , EPH , EPHT , EPHT1

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EphA1 antibody [C1C3-2] used in Western Blot (WB). GTX111449

GTX111449 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA1 antibody [C1C3-2] (GTX111449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-EphA1 antibody [C1C3-2] used in Western Blot (WB). GTX111449

GTX111449 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX111449 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169