Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX111449 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA1 antibody [C1C3-2] (GTX111449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.