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EphA10 antibody

Anti-EphA10 antibody used in Western Blot (WB). GTX85273

Cat. No. GTX85273

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all EphA10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

EphA10 antibody was raised against a 14 amino acid synthetic peptide of near the amino terminus of human EphA10.The immunogen is located within amino acids 120 - 170 of EphA10.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor A10

Background

Eph receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell–cell communication regulating cell attachment, shape, and mobility of neuronal and endothelial cells in central nervous system function and in development. Eph receptors can be divided into two subgroups: EphA and EphB. In mammals, the EphA class consists of eight members (EphA 1-7 and 10) that in general bind to ephrin-A members linked to the cell membrane through a glycosylphosphatidylinositol linkage. The EphB class consists of six members (EphB 1-6) that in general bind ephrin-B members that transverse the cell membrane. The Ephrin / EPH signaling pathway networks with the WNT signaling pathway during embryogenesis, tissue regeneration, and carcinogenesis. Recent studies show that Eph/EFN might be relevant in normal B-cell biology and could represent new potential prognostic markers and therapeutic targets for CLL.

Database

DATA IMAGES

Anti-EphA10 antibody used in Western Blot (WB). GTX85273

GTX85273 WB Image

WB analysis of 293 cell lysate using GTX85273 EphA10 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399