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EphA3 antibody [N1N3]

Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067
Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067
Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067
Anti-EphA3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114067
Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067

Cat. No. GTX114067

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EphA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Eph Receptor A3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164961

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor A3 , EK4 , ETK , ETK1 , HEK , HEK4 , TYRO4

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067

GTX114067 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067

GTX114067 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067

GTX114067 WB Image

Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EphA3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114067

GTX114067 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Eph Receptor A3(GTX114067) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EphA3 antibody [N1N3] used in Western Blot (WB). GTX114067

GTX114067 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169