EphA4 antibody [N3C2-2], Internal

Name: EphA4 antibody [N3C2-2], Internal
Brand: GeneTex
SKU: GTX111602
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-EphA4 antibody [N3C2-2], Internal used in Western Blot (WB). GTX111602

Cat. No. GTX111602

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all EphA4 products

View product citations for antibody GTX111602 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

HeLa , 293T , mouse brain , rat brain , mouse hippocampus , DDDDK-tagged EPHA4-transfected 293T

Product Note

Based on internal testing result, this antibody may cross react with EphA5, EphA6, and EphA7.

Predict Reactivity

Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EphA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168956

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor A4 , EK8 , HEK8 , SEK , TYRO1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq]

Database

Gene ID: 2043 EPHA4
UniProt: P54764 EPHA4

Research Area

DATA IMAGES

GTX111602 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2-2], Internal (GTX111602) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111602 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX111602 diluted at 1:1000

GTX111602 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody (GTX111602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111602 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX111602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111602 WB Image

Eph receptor A4 antibody [N3C2-2], Internal detects Eph receptor A4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Eph receptor A4 antibody [N3C2-2], Internal (GTX111602) diluted by 1:1000

REFERENCE

Application Reference

Li Z et al. Front Cell Neurosci 2020; 14 : 144 MicroRNA-124 Overexpression in Schwann Cells Promotes Schwann Cell-Astrocyte Integration and Inhibits Glial Scar Formation Ability.

Application : WB

Reactivity : Rat

Hua KT et al. Cancer Res 2017; (Epub) MicroRNA-519d promotes melanoma progression by downregulating EphA4.

Application : IHC-P

Reactivity : Mouse

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