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EphA4 antibody [N3C2], Internal

Overexpression
GTX104109_40583_WB_19011506_715.jpg
GTX104109_40583_WB_brain_19011506_483.jpg
GTX104109_40583_20170518_WB_M_R_19011506_367.jpg
GTX104109_40583_20180727_WB_B_19011506_756.jpg
GTX104109_40583_20170608_WB_M_hippocampus_19011506_739.jpg
GTX104109_40583_WB_B_19011506_401.jpg

Cat No. GTX104109

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , 3xFlag-human EPHA4-transfected 293T , human brain , mouse brain , rat brain , mouse hippocampus , DDDDk-tagged EPHA4-transfected 293T

Predict Reactivity

Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Eph receptor A4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eph Receptor A4,Ek8,Hek8,Sek,Tyro1,Epha4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104109_40583_WB_19011506_715.jpg

GTX104109 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX104109 diluted at 1:1000

GTX104109_40583_WB_brain_19011506_483.jpg

GTX104109 WB Image

Eph receptor A4 antibody detects Eph receptor A4 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Eph receptor A4 antibody (GTX104109) diluted by 1:500.

GTX104109_40583_20170518_WB_M_R_19011506_367.jpg

GTX104109 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody (GTX104109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104109_40583_20180727_WB_B_19011506_756.jpg

GTX104109 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX104109) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104109_40583_20170608_WB_M_hippocampus_19011506_739.jpg

GTX104109 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX104109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104109_40583_WB_B_19011506_401.jpg

GTX104109 WB Image

Overexpression

Eph receptor A4 antibody [N3C2], Internal detects Eph receptor A4 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 5 μg whole cell extract of 3xFlag-human EPHA4-transfected 293T cells
5 % SDS-PAGE
Eph receptor A4 antibody [N3C2], Internal (GTX104109) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169