APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 , human brain , mouse brain , rat brain , mouse hippocampus , DDDDk-tagged EPHA4-transfected 293T
Predict Reactivity
Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human EphA4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11170602
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EPH receptor A4 , EK8 , HEK8 , SEK , TYRO1
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX104109 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX104109) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104109 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody (GTX104109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104109 WB Image
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX104109 diluted at 1:1000
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GTX104109 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (GTX104109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104109 WB Image
Eph receptor A4 antibody detects Eph receptor A4 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Eph receptor A4 antibody (GTX104109) diluted by 1:500.
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REFERENCE
There are currently no references for EphA4 antibody [N3C2], Internal (GTX104109). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EphA4 antibody [N3C2], Internal (GTX104109). Be the first to share your experience with this product.