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EphA4 antibody

Anti-EphA4 antibody used in Western Blot (WB). GTX25396
Anti-EphA4 antibody used in Western Blot (WB). GTX25396
Anti-EphA4 antibody used in Western Blot (WB). GTX25396
Anti-EphA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX25396
Anti-EphA4 antibody used in Western Blot (WB). GTX25396
Anti-EphA4 antibody used in Western Blot (WB). GTX25396

Cat. No. GTX25396

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
200 μl ($399)
See all EphA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

NCI-H460 cell lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 875-904 amino acids from the C-terminal region of human EphA4.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor A4 , EK8 , HEK8 , SEK , TYRO1

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-EphA4 antibody used in Western Blot (WB). GTX25396

GTX25396 WB Image

WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 (Lane 2) using GTX25396 EphA4 antibody.

Anti-EphA4 antibody used in Western Blot (WB). GTX25396

GTX25396 WB Image

WB analysis of HeLa, HUVEC cell using GTX25396 EphA4 antibody.
Loading : 20ug per lane
Dilution : 1:1000

Anti-EphA4 antibody used in Western Blot (WB). GTX25396

GTX25396 WB Image

WB analysis of HeLa, NCI-H460, HUVEC cell using GTX25396 EphA4 antibody.
Loading : 20ug per lane
Dilution : 1:1000

Anti-EphA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX25396

GTX25396 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX25396 EphA4 antibody.

Anti-EphA4 antibody used in Western Blot (WB). GTX25396

GTX25396 WB Image

WB analysis of HeLa, NCI-H460, NIH3T3 cell and human ovary tissue lysate using GTX25396 EphA4 antibody.
Loading : 35ug per lane
Dilution : 1:1000

Anti-EphA4 antibody used in Western Blot (WB). GTX25396

GTX25396 WB Image

WB analysis of NCI-H460 cell lysate using GTX25396 EphA4 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399