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EphA5 antibody

Anti-EphA5 antibody used in Western Blot (WB). GTX66237
Anti-EphA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66237

Cat. No. GTX66237

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-130 of human EPHA5 (NP_004430.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

EPH receptor A5 , CEK7 , EHK-1 , EHK1 , EK7 , HEK7 , TYRO4

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-EphA5 antibody used in Western Blot (WB). GTX66237

GTX66237 WB Image

WB analysis of various sample lysates using GTX66237 EphA5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-EphA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66237

GTX66237 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66237 EphA5 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399