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EphA8 antibody

Cat No. GTX10615

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Mouse
Package
50 μg ($549)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/ml

Note :

ELISA
using Trypsin-digested, human and animal tissue sections

Not tested in other applications.

Calculated MW

111 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

purified recombinant mouse EphA8 extracellular domain.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Eph receptor A8 , AW047546 , Eek , Hek3 , mKIAA1459

Cellular Localization

Cell membrane

Background

EphA8, also known as Eek and Hek3, is a member of the Eph receptor family, which binds members of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine. The calculated molecular mass of the reduced mouse EphA8/Fc monomer is 84.2 kDa, but as a result of glycosylation, recombinant EphB8/Fc migrates as an approximately 100-120 kDa protein under reducing conditions in SDS-PAGE. Ligand recognition and binding leads to activation of the intrinsic kinase activity. EphA8 binds to Ephrin-A2, Ephrin-A3, and Ephrin-A5. Full length human and rat homolgs have not been successfully cloned. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation.The ephrin ligands and eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Database

Research Area

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 549