APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
This EphB2 antibody is generated from rabbits immunized with a recombinant human EphB2 protein.
Purification
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EPH receptor B2 , CAPB , DRT , EK5 , EPHT3 , ERK , Hek5 , PCBC , Tyro5
Cellular Localization
Cell membrane; Single-pass type I membrane protein,Cell projection, dendrite
Background
This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Database
Research Area
DATA IMAGES
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GTX81348 WB Image
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB2 (Lane 2) using GTX81348 EphB2 antibody.
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GTX81348 IHC-P Image
IHC-P analysis of human lung carcinoma using GTX81348 EphB2 antibody.
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GTX81348 FACS Image
FACS analysis of NCI-H460 cells using GTX81348 EphB2 antibody.
Top histogram : negative control
Bottom histogram : NCI-H460 cells
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REFERENCE
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REVIEW
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