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EphB4 antibody [5B8F7]

Anti-EphB4 antibody [5B8F7] used in Western Blot (WB). GTX83333
Anti-EphB4 antibody [5B8F7] used in IHC (Paraffin sections) (IHC-P). GTX83333

Cat. No. GTX83333

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B8F7

Isotype

 IgG2a

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all EphB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Product Note

It is only specific to EPHB4

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of EphB4 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPH receptor B4 , CMAVM2 , HFASD , HTK , LMPHM7 , MYK1 , TYRO11

Cellular Localization

Cell membrane

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EphB4 antibody [5B8F7] used in Western Blot (WB). GTX83333

GTX83333 WB Image

WB analysis of truncated EphB4 recombinant protein using GTX83333 EphB4 antibody [5B8F7].

Anti-EphB4 antibody [5B8F7] used in IHC (Paraffin sections) (IHC-P). GTX83333

GTX83333 IHC-P Image

IHC-P analysis of human pancreas carcinoma (left) and breast carcinoma (right) tissue using GTX83333 EphB4 antibody [5B8F7].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399