GeneTex
United States (US)

EphB4 antibody [N1N2], N-term

GTX108595_39932_20180316_WB_KO_watermark_19011507_425.jpg
GTX108595_WB_19011507_283.jpg

Cat No. GTX108595

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Eph receptor B4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eph Receptor B4,Hfasd,Htk,Myk1,Tyro11,Ephb4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108595_39932_20180316_WB_KO_watermark_19011507_425.jpg

GTX108595 WB Image

Wild-type (WT) and EphB4 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphB4 antibody [N1N2], N-term (GTX108595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX108595_WB_19011507_283.jpg

GTX108595 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
Eph receptor B4 antibody
GTX108595 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf