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EphB4 antibody

Anti-EphB4 antibody used in Western Blot (WB). GTX66654
Anti-EphB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX66654

Cat. No. GTX66654

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all EphB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 580-630 of Human EphB4.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

EPH receptor B4 , CMAVM2 , HFASD , HTK , LMPHM7 , MYK1 , TYRO11

Cellular Localization

Cell membrane

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EphB4 antibody used in Western Blot (WB). GTX66654

GTX66654 WB Image

WB analysis of various samples using GTX66654 EphB4 antibody.
Lane1 : Hcc827 whole cell lysate
Lane2 : LO2 whole cell lysate
Lane3 : The Kidney tissue lysate of Mouse
Lane4 : The Kidney tissue lysate of Rat
Dilution : 1:500

Anti-EphB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX66654

GTX66654 IHC-P Image

IHC-P analysis of human brain tissue using GTX66654 EphB4 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399