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Ephrin A2 antibody [4C3]

Anti-Ephrin A2 antibody [4C3] used in Flow cytometry (FACS). GTX84590
Anti-Ephrin A2 antibody [4C3] used in Western Blot (WB). GTX84590
Anti-Ephrin A2 antibody [4C3] used in Flow cytometry (FACS). GTX84590
Anti-Ephrin A2 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84590

Cat. No. GTX84590

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C3

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all Ephrin A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ephrin A2 , ELF-1 , EPLG6 , HEK7-L , LERK-6 , LERK6

Cellular Localization

Cell membrane

Background

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ephrin A2 antibody [4C3] used in Flow cytometry (FACS). GTX84590

GTX84590 FACS Image

FACS analysis of HEK293T cells transfected with either Ephrin A2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84590 Ephrin A2 antibody [4C3].

Anti-Ephrin A2 antibody [4C3] used in Western Blot (WB). GTX84590

GTX84590 WB Image

WB analysis of HEK293T cells transfected with Ephrin A2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84590 Ephrin A2 antibody [4C3].
Loading : 5 ug per lane

Anti-Ephrin A2 antibody [4C3] used in Flow cytometry (FACS). GTX84590

GTX84590 FACS Image

FACS analysis of Jurkat cells using GTX84590 Ephrin A2 antibody [4C3].
Red : Primary antibody
Blue : Negative control antibody

Anti-Ephrin A2 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84590

GTX84590 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Ephrin A2 plasmid using GTX84590 Ephrin A2 antibody [4C3].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519