GeneTex
United States (US)

Ephrin A2 (cleaved Asn188) antibody

EFNA2-cleaved-Asn188-antibody-GTX86940-WB-1_18121410_900.jpg

Cat No. GTX86940

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

21 kDa. ( Note )

Specificity/Sensitivity

EFNA2 (Cleaved-Asn188) Antibody detects endogenous levels of fragment of activated EFNA2 resulting from cleavage adjacent to Asn188.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human EFNA2.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ephrin A2,Elf-1,Eplg6,Hek7-L,Lerk-6,Lerk6,Efna2

Background

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq]

Database

Research Area

DATA IMAGES
EFNA2-cleaved-Asn188-antibody-GTX86940-WB-1_18121410_900.jpg

GTX86940 WB Image

Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using EFNA2 (Cleaved-Asn188) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339