GeneTex
United States (US)

Ephrin A5 antibody

Anti-Ephrin A5 antibody used in Western Blot (WB). GTX34361

Cat No. GTX34361

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

Ephrin-A5 Polyclonal Antibody detects endogenous levels of Ephrin-A5 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from the Internal region of human Ephrin-A5 at AA range: 1-80.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ephrin A5,Af1,Efl5,Eplg7,Glc1M,Lerk7,Rags,Efna5

Background

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Ephrin A5 antibody used in Western Blot (WB). GTX34361

GTX34361 WB Image

Western Blot (WB) analysis of specific cells using Ephrin-A5 Polyclonal Antibody.

Package List Price ($)
$ 319