GeneTex
United States (US)

Ephrin B2 antibody [N1C1]

GTX105582_WB_19011506_417.jpg
GTX105582_39932_20170511_WB_M_R_19011506_205.jpg

Cat No. GTX105582

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

Predict Reactivity

Cat, Dog, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ephrin-B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EPLG5 antibody, HTKL antibody, Htk-L antibody, LERK5 antibody, MGC126226 antibody, MGC126227 antibody, MGC126228 antibody, EFNB2 antibody, ephrin-B2 antibody, eph-related receptor tyrosine kinase ligand 5 antibody, HTK ligand antibody, LERK-5 antibody, ligand of eph-related kinase 5 antibody

Cellular Localization

Membrane

Background

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105582_WB_19011506_417.jpg

GTX105582 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX105582 diluted at 1:1000

GTX105582_39932_20170511_WB_M_R_19011506_205.jpg

GTX105582 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ephrin B2 antibody (GTX105582) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169