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Ephrin B2 antibody

Cat. No. GTX27043

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Mouse
Package
50 μg ($399)
See all Ephrin B2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 5-15 μg/ml
FACS 0.25 μg/1 × 10⁶ cells
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 5% Trehalose

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

recombinant mouse ephrin-B2, expressed in NSO cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ephrin B2 , ELF-2 , Epl5 , Eplg5 , Htk-L , LERK-5 , Lerk5 , NLERK-1

Cellular Localization

Cell membrane

Background

Ephrin-B2 (the antigen to this antibody) is also known as Htk-L, LERK-5, NLERK-1 and ELF-2 and binds EphA4, EphB1, EphB2, EphB3, and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells (ref 1), bone marrow fibroblasts(ref 2), activated melanocytes and melanoma cells (ref 3), and pre-artery endothelial cells (ref 4). To date, at least 14 members of the Eph receptor family and a family of 8 ligands have been identified. Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses, ephrin-A and ephrin-B. Ligands .

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399