GeneTex
United States (US)

Epiregulin antibody

GTX16256_1363_WB_18121410_884.jpg
GTX16256_1364_WB_18121410_366.jpg
GTX16256_651_IHC-P_18121410_536.jpg
GTX16256_1365_WB_18121410_976.jpg
GTX16256_1366_WB_18121410_848.jpg
GTX16256_652_IHC-P_18121410_797.jpg
GTX16256_23_FACS_18121410_354.jpg

Cat No. GTX16256

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

19 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epiregulin,Epr,Er,Ep,Ereg

Background

Epiregulin is a member of the epidermal growth factor family. Epiregulin can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX16256_1363_WB_18121410_884.jpg

GTX16256 WB Image

WB analysis of various samples using GTX16256 Epiregulin antibody.
Lane 1: A549 whole cell lysates
Lane 2: human placenta lysates
Loading : 20 µg per lane
Dilution : 1:1000

GTX16256_1364_WB_18121410_366.jpg

GTX16256 WB Image

WB analysis of various samples using GTX16256 Epiregulin antibody.
Lane 1: HepG2 whole cell lysates
Lane 2: A549 whole cell lysates
Lane 3: NCI-H1299 whole cell lysates
Lane 4: U-87 MG whole cell lysates
Loading : 20 µg per lane
Dilution : 1:2000

GTX16256_651_IHC-P_18121410_536.jpg

GTX16256 IHC-P Image

IHC-P analysis of human brain tissue using GTX16256 Epiregulin antibody.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

GTX16256_1365_WB_18121410_976.jpg

GTX16256 WB Image

WB analysis of A549 whole cell lysate using GTX16256 Epiregulin antibody.
Loading : 20 µg per lane
Dilution : 1:2000

GTX16256_1366_WB_18121410_848.jpg

GTX16256 WB Image

WB analysis of U-87 MG whole cell lysate using GTX16256 Epiregulin antibody.
Loading : 20 µg per lane
Dilution : 1:2000

GTX16256_652_IHC-P_18121410_797.jpg

GTX16256 IHC-P Image

IHC-P analysis of human brain tissue using GTX16256 Epiregulin antibody.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

GTX16256_23_FACS_18121410_354.jpg

GTX16256 FACS Image

FACS analysis of HepG2 cells using GTX16256 Epiregulin antibody.
Red : Epiregulin
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25