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Eps15 antibody [C3], C-term

Anti-Eps15 antibody [C3], C-term used in Western Blot (WB). GTX108486
Anti-Eps15 antibody [C3], C-term used in Western Blot (WB). GTX108486

Cat. No. GTX108486

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Eps15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

Raji , K562 , BCL-1

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Eps15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036870

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor pathway substrate 15 , AF-1P , AF1P , MLLT5

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 2: Early endosome membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Eps15 antibody [C3], C-term used in Western Blot (WB). GTX108486

GTX108486 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX 108486 diluted at 1:500

Anti-Eps15 antibody [C3], C-term used in Western Blot (WB). GTX108486

GTX108486 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
5% SDS PAGE
GTX108486 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169