Equine Encephalitis Virus nsP2 antibody [HL1920]

Cat. No. GTX637669

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1920
Isotype	IgG
Application	WB, ICC/IF, IHC-P (cell pellet)
Reactivity	Venezuelan equine encephalitis virus, Eastern equine encephalitis virus, Western equine encephalitis virus
Package	100 μl ($399), 25 μl ($169)

See all Equine Encephalitis Virus nsP2 products

Datasheet

View product citations for antibody GTX637669 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

Product Note

This antibody detects nsP2 protein of VEEV, EEEV, and WEEV.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Venezuelan equine encephalitis virus

Immunogen

Recombinant fragment of Venezuelan Equine Encephalitis Virus nsP2. (Strain P676)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VEEV virus nsp2,WEEV virus nsp2,EEEV virus nsp2,Venezuelan equine encephalitis virus nsp2,Western equine encephalitis virus nsp2,Eastern equine encephalitis virus nsp2,

Database

UniProt: P36328

DATA IMAGES

GTX637669 ICC/IF Image

Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP2 antibody [HL1920] antibody (GTX637669).
Sample: Multiple virus infected cells slide.
Green: Venezuelan Equine Encephalitis Virus nsP2 antibody [HL1920] antibody (GTX637669) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637669 IHC-P (cell pellet) Image

Equine Encephalitis Virus nsP2 antibody [HL1920] detects Equine Encephalitis Virus nsP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mock and VEEV nsP2 (Venezuelan equine encephalitis virus) - transfected 293T cell pellet.
Green: Equine Encephalitis Virus nsP2 stained by Equine Encephalitis Virus nsP2 antibody [HL1920] (GTX637669) diluted at 1:5000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Equine Encephalitis Virus nsP2 antibody [HL1920] (GTX637669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Equine Encephalitis Virus nsP2 antibody [HL1920]

Cat. No. GTX637669

Host

Clonality

Clone Name

Isotype

Application

Reactivity