Esrp-1/2 antibody [23A7.C9]

WB analysis of transfected 293T whole cell lysate using GTX48760 Esrp-1/2 antibody [23A7.C9].
Lane 1 : GFP
Lane 2 : Espr-1
Lane 3 : Esrp-2
Loading : 5 μg
Dilution : 1:1000

Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 μg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.