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Estrogen Receptor alpha antibody [1F3]

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

Cat No. GTX70171

Host

Mouse

Clonality

Monoclonal

Clone Name

1F3

Isotype

IgG1

Application

WB, IHC-Fr, FACS, IP

Reactivity

Human, Rabbit
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

MCF-7 , DDDDK-tagged ESR1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding amino acids 1-190 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Estrogen Receptor 1 , Er , Esr , Esra , Estrr , Era , Nr3A1 , Esr1

Cellular Localization

Nuclear

Background

The Estrogen Receptor (ER) is a 65a protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Whole cell extract (60 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX70171) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:500 and competitor's antibody (MA1-310) diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
The observed M.W. is based on the publication: PMID: 26397740

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329