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Estrogen Receptor alpha antibody [1F3]

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

Cat No. GTX70171

Host

Mouse

Clonality

Monoclonal

Clone Name

1F3

Isotype

IgG1

Application

WB, IHC-Fr, FACS, IP

Reactivity

Human, Rabbit
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

MCF-7 , DDDDK-tagged ESR1-transfected 293T

Product Note

This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding amino acids 1-190 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370361

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:500 and competitor's antibody (MA1-310) diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
The observed M.W. is based on the publication: PMID: 26397740

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Whole cell extract (60 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX70171) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 24724101

REFERENCE

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Application Reference
Clone Reference
SDS
PBS.pdf