GeneTex

  • Country / Location Selection

United States (US)

Estrogen Receptor alpha antibody [1F3]

Comparison Orthogonal Overexpression
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171
Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

Cat. No. GTX70171

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F3

Isotype

 IgG1

Application

 WB, IHC-Fr, FACS, IP

Reactivity

 Human, Rabbit
Package
100 μl ($399)
See all Estrogen Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

MCF-7 , DDDDK-tagged ESR1-transfected 293T

Product Note

This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding amino acids 1-190 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Whole cell extract (60 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (GTX70171) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 24724101

Anti-Estrogen Receptor alpha antibody [1F3] used in Western Blot (WB). GTX70171

GTX70171 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [1F3] (GTX70171) diluted at 1:500 and competitor's antibody (MA1-310) diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
The observed M.W. is based on the publication: PMID: 26397740

REFERENCE

Application Reference

Chang YC et al. Front Cell Dev Biol 2020; 8 : 485 Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer Cells.
Application : WB
Reactivity : Human
Ma L et al. Lung Cancer Manag 2019; 8 (1) : LMT04 Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma.
Daurio NA et al. Cancer Res 2016; (Epub) ampk activation and metabolic reprogramming by * through estrogen receptor-independent mechanisms suggests new uses for this therapeutic modality in cancer treatment.
Application : WB
Reactivity : Human
Mahajan K et al. J Biol Chem 2014; 289 (41) : 28179-91 ACK1 tyrosine kinase interacts with histone demethylase KDM3A to regulate the mammary tumor oncogene HOXA1.
Application : WB
Reactivity : Human
Ivanova MM et al. Mol Cell Endocrinol 2009; 305 (43102) : 43455 Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Estrogen Receptor alpha antibody [1F3] (GTX70171). Be the first to share your experience with this product.
Submit a Review
Application Reference
Chang YC et al. Front Cell Dev Biol 2020; 8:485 Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer Cells.
Application : WB
Reactivity : Human
Ma L et al. Lung Cancer Manag 2019; 8 (1):LMT04 Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma.
Daurio NA et al. Cancer Res 2016; (Epub) ampk activation and metabolic reprogramming by * through estrogen receptor-independent mechanisms suggests new uses for this therapeutic modality in cancer treatment.
Application : WB
Reactivity : Human
Mahajan K et al. J Biol Chem 2014; 289 (41):28179-91 ACK1 tyrosine kinase interacts with histone demethylase KDM3A to regulate the mammary tumor oncogene HOXA1.
Application : WB
Reactivity : Human
Ivanova MM et al. Mol Cell Endocrinol 2009; 305 (43102):43455 Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells.
Application : WB
Reactivity : Human
Srilatha B et al. Urology 2004; 63 (2):382-6 Estrogen and phytoestrogen predispose to erectile dysfunction: do ER-alpha and ER-beta in the cavernosum play a role?
Application : IHC-Fr
Reactivity : Rabbit
Clone Reference
Sun M et al. Mol Cancer Ther 2008; 7 (3):464-73 Curcumin (diferuloylmethane) alters the expression profiles of microRNAs in human pancreatic cancer cells.
Application : FACS
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations