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Estrogen Receptor alpha antibody [33]

Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746
Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746
Anti-Estrogen Receptor alpha antibody [33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22746

Cat. No. GTX22746

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 33

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, Gel supershift assays, IHC

Reactivity

 Human, Mouse, Rat, Bovine, Japanese Medaka, Primate, Avian
Package
50 μg ($399)
See all Estrogen Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 4 μg/ml
ICC/IF 10 μg/ml
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
Gel supershift assays Assay dependent
IHC 1:20 - 1:100
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Product Note

This antibody detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. It detects ER alpha and does not cross react with ER beta.

PROPERTIES

Form

Liquid

Buffer

PBS, 30% Glycerol, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_370351

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746

GTX22746 WB Image

WB analysis of full-length human ER alpha recombinant protein and ER beta protein using GTX22746 Estrogen Receptor alpha antibody [33] at 4 μg/ml (left panel) or GTX25786 Estrogen Receptor beta antibody at 1:2000 (right panel).

Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746

GTX22746 WB Image

WB analysis of 25 ug of the indicated whole cell lysates using GTX22746 Estrogen Receptor alpha antibody [33].
Dilution : 4 μg/ml

Anti-Estrogen Receptor alpha antibody [33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22746

GTX22746 ICC/IF Image

ICC/IF analysis of T-47D cells using GTX22746 Estrogen Receptor alpha antibody [33].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 10 μg/ml overnight at 4ºC

REFERENCE

Application Reference

Kao TL et al. Theranostics 2018; 8 (10) : 2672-2682 Estrogen receptors orchestrate cell growth and differentiation to facilitate liver regeneration.
Kao T.L. et al. Theranostics 2018; 8 (10) : 2672-2682 Estrogen receptors orchestrate cell growth and differentiation to facilitate liver regeneration.
Application : IP
Reactivity : Mouse
Massinen S et al. Hum Mol Genet 2009; 18 (15) : 329785 Functional interaction of DYX1C1 with estrogen receptors suggests involvement of hormonal pathways in dyslexia.

Clone Reference

Tam A et al. PLoS One 2014; 9 (6):e100633 * increases mucus synthesis in bronchial epithelial cells.
Application : IHC-P
Reactivity : Human
Mahmoodzadeh S et al. J Biol Chem 2009; 284 (37):24705-14 Nuclear factor-kappaB regulates estrogen receptor-alpha transcription in the human heart.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399