GeneTex
  • Country / Location Selection

United States (US)

Estrogen Receptor alpha antibody [33]

Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746
Anti-Estrogen Receptor alpha antibody [33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22746
Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746

Cat No. GTX22746

Host

Mouse

Clonality

Monoclonal

Clone Name

33

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP, Gel supershift assays, IHC

Reactivity

Human, Mouse, Rat, Bovine, Fish, Primate, Avian
Package
50 μg ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 4 μg/ml
ICC/IF 10 μg/ml
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
Gel supershift assays Assay dependent
IHC 1:20 - 1:100
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Specificity/Sensitivity

This antibody detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. It detects ER alpha and does not cross react with ER beta.

PROPERTIES

Form

Liquid

Buffer

PBS, 30% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746

GTX22746 WB Image

WB analysis of full-length human ER alpha recombinant protein and ER beta protein using GTX22746 Estrogen Receptor alpha antibody [33] at 4 μg/ml (left panel) or GTX25786 Estrogen Receptor beta antibody at 1:2000 (right panel).

Anti-Estrogen Receptor alpha antibody [33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22746

GTX22746 ICC/IF Image

ICC/IF analysis of T-47D cells using GTX22746 Estrogen Receptor alpha antibody [33].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 10 μg/ml overnight at 4ºC

Anti-Estrogen Receptor alpha antibody [33] used in Western Blot (WB). GTX22746

GTX22746 WB Image

WB analysis of 25 ug of the indicated whole cell lysates using GTX22746 Estrogen Receptor alpha antibody [33].
Dilution : 4 μg/ml

REFERENCE

REVIEW

There are currently no reviews for Estrogen Receptor alpha antibody [33] (GTX22746). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 259